cd54 icam 1 mouse antibody Search Results


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R&D Systems nephrin
(A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized <t>with</t> <t>caveolin</t> 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with <t>nephrin</t> (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.
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(A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized <t>with</t> <t>caveolin</t> 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with <t>nephrin</t> (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.
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(A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized <t>with</t> <t>caveolin</t> 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with <t>nephrin</t> (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.
Anti Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized <t>with</t> <t>caveolin</t> 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with <t>nephrin</t> (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.
Anti Cd54, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized <t>with</t> <t>caveolin</t> 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with <t>nephrin</t> (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.
Anti Cd54 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd54 icam 1 antibody
(A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized <t>with</t> <t>caveolin</t> 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with <t>nephrin</t> (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.
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Sino Biological anti icam1 ab
(A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized <t>with</t> <t>caveolin</t> 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with <t>nephrin</t> (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.
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R&D Systems rat anti mouse icam1
(A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized <t>with</t> <t>caveolin</t> 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with <t>nephrin</t> (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.
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Miltenyi Biotec icam 1 cd54 pe antibodies
(A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized <t>with</t> <t>caveolin</t> 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with <t>nephrin</t> (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.
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Image Search Results


(A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized with caveolin 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with nephrin (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.

Journal: Pediatric nephrology (Berlin, Germany)

Article Title: Glomerular endothelial cells and podocytes can express CD80 in patients with minimal change disease during relapse

doi: 10.1007/s00467-020-04541-3

Figure Lengend Snippet: (A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized with caveolin 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with nephrin (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.

Article Snippet: Reagents and antibodies The following primary antibodies were used CD80 (AF740, R&D) at 1:100 dilution; CD80 (MAB140, clone 37711, R&D) at 1:20; caveolin 1 (D46G3, #3267, Cell Signaling) at 1:800, nephrin (provided by Verma R), ICAM-1 (AF796, R&D) at 1:1000, synaptopodin (10R-2373, Fitzgerald) at 1:20.

Techniques: Immunostaining, Silver Staining, Electron Microscopy